TABLE 2.
Primers and probes used in mRNA expression analysis
| Gene name | Sequencea (5′–3′) |
|
|---|---|---|
| Forward | Reverse | |
| PSMC1 | CATGGCCACAAACCGAAT | ATCAGGCAGGGGGAACTC |
| PSMC4 | GGAAGACCATGTTGGCAAAG | AAGATGATGGCAGGTGCATT |
| PSMC6 | GGCAGATCGTGGGTGAAG | CGACGACAACCCACAACA |
| PSMD3 | GCTGTGCAGGGCTTCTTC | GGTGTCGACGCAGCTTTT |
| PSMD6 | GGCAAAGGCCGAGTACCT | ACCCAGGGCCACAGTTTT |
| PSMD14 | CCGTGCTGGAGTTCCAAT | TGCCTCCACACTGACACC |
| PSMB4 | TCTCGGCCAGATGGTGAT | CACATAACCGAGGAAGCT |
| PSMB7 | CGGCTGTGTCGGTGTATG | GCCAGTTTTCCGGACCTT |
| OGT | CCGACTTTGGGAATCATCCT | GCTTCTGCCATCACCTTCAC |
| NRF1 | TGGAACAGCAGTGGCAAGATCTCA | GGCACTGTACAGGATTTCACTTGC |
| HCF-1 | GTACAACACGGCAACCAACC | CCATACTCCACCATCCCACC |
| HPRT | CCGGCTCCGTTATGGC | GGTCATAACCTGGTTCATCATCA |
| HPRT probe | FAM-CGCAGCCCTGGCGTCGTGATTA-TAMRA | |
a
FAM, 6-carboxyfluorescein; TAMRA, 6-carboxytetramethylrhodamine.