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. 2018 Jul 23;115(32):E7568–E7577. doi: 10.1073/pnas.1803062115

Fig. 1.

Fig. 1.

Single-cell RNA sequencing of transgenic mosquitoes. A PPO6::RFP transgenic mosquito strain was used for isolation of blood cells. (A) An RFP-expressing hemocyte (red) obtained by perfusion of a female adult mosquito. (B) Only a proportion of adult mosquito blood cells display RFP expression (red, arrow) whereas other cells of sizes suggestive of hemocytes do not (arrowheads). (C) FACS sorting of RFP+ (R+) Hoechst+ (H+) blood cells from hemolymph of perfused PPO6::RFP females. (D) Representative image of a sorted cell. (E) The pipeline developed for the study of mosquito blood cells based on single-cell RNA sequencing. Cells were sorted into a 96-well plate, processed according to the SMART-Seq2 protocol, and sequenced in a HiSeq Illumina platform. Image courtesy of Suzana Zakovic (Max Planck Institute for Infection Biology, Berlin). (F) Scatter plot for the average normalized read counts from pools and single cells. r2 indicates Pearson correlation. (G) Venn diagram of genes detected in single cells and pools (normalized count ≥ 1). (Scale bars: A, 10 µm; B, 20 µm; D, 5 µm.) DNA is stained with DAPI (A and B) and Hoechst (D).