Fig. 3.

Characterization of PPO6^high and PPO6^low cell subpopulations. (A) Violin plots of the expression of putative population and panhemocyte markers. (B) RNA-FISH validation of identified PPO6^high and PPO6^low cell subpopulations in perfused cells based on markers shown in A. Cells were classified as PPO6^high (Upper) or PPO6^low (Lower) according to the expression of PPO6 (red). Arrows indicate lower PPO6 signal. (C–C‴) PPO6^high and PPO6^low cell subpopulations can also be seen as tissue-resident blood cells attached to the inner abdominal wall of female mosquitoes. Arrow and arrowhead indicate PPO6^high and PPO6^low cell subpopulations, respectively. C‴ shows higher amplification of the C′ boxed area. (Scale bars: B, 5 µm; C and C′, 50 µm; C″ and C‴, 5 µm.) DNA is stained with DAPI.