Fig. 6.

Both Eda signaling and the BAF complex contribute to chromatin remodeling of Eda target genes. (A) Chromatin was extracted from Edar⁻ or Edar⁺ cells treated with Eda CM, and then digested with DNase I (from 0 to 5 U/mL). Undigested DNA was amplified by qPCR with primers targeting Ltb loci. A distal Ctl locus was an inner control. Signals for DNase-untreated chromatin are normalized to 100%. (B) As in A, except primers targeting Ctl and Tnfrsf9 loci were used. (C) CHIP-qPCR assays show the binding activity of Ring1b in Ltb and Tnfrsf9 loci in the indicated cells and treatments. (D) As in C, except that Ezh2 CHIP was carried out. Error bars indicate mean ± SEM for triplicates. *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001 by Student’s t test.