Fig. 3.

HCV genome replication is less dependent on miR-122 in cells lacking DUSP11. (A) sgJFH1-Neo replicon colony formation assays in parental HEK293 cells and DUSP11 knockout cells (D11-KO-5). Quantitation of both the relative area colonized and relative colony counts with transfection of sgJFH1-Neo RNA is presented (Left). The mean ± SEM of three individual experiments is presented. Example wells with stained colonies from the indicated cell lines transfected with the indicated RNA are displayed (Right). (B) sgJFH1-Rluc replicon luciferase assay in parental Huh7 cells and two independent DUSP11 knockout clones (D11-KO-8 and D11-KO-9) treated with irrelevant control anti-miR (antiNC) or miR-122–specific anti-miR (anti122). Luciferase assays were performed at the indicated times post replicon RNA transfection. The mean of three individual experiments ± SEM is presented. Statistical significance was assessed by Student’s t test and is indicated as follows: *P < 0.05, **P < 0.01.