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. 2018 Jul 17;115(32):E7486–E7494. doi: 10.1073/pnas.1720342115

Fig. 3.

Fig. 3.

Basal nucleotide exchange in the absence of actin. (A and B) Basal single-ATP turnover by stopped-flow mix of 0.2 µM HMM (A) or 0.4 µM S1 (B), mixed with 4.0 μM mant-ATP and then chased with 2.0 mM MgATP. Blue and violet traces indicate 30 µM mava. (C–E) Amplitudes (C) and rates (D and E) of the two-exponential fits of A and B. (F and G) Basal single ADP dissociation from HMM (F) or S1 (G), mixed with 4.0 μM mant-ADP and then chased with 2.0 mM MgATP. (H–J) Amplitudes (H) and rates (I and J) of the two-exponential fits of F and G. n = 9 replicates. Two-exponential fits are reported in SI Appendix, Table S1. Error bars indicate SEM. **P ≤ 0.01, ***P ≤ 0.001; N.S. (not significant), P > 0.05.