Fig. 1.

Analysis of Slc30a8 RNA and protein in islets from male R138X mice on chow diet. (A) Slc30a8 RNA in situ hybridization of pancreatic islets isolated from wild-type, knockout, and R138X mice. KO islets were used as negative control. Red, glucagon RNA; green, insulin RNA; white, Slc30a8 RNA. (B) Quantification of islet Slc30a8 RNA levels using qPCR analysis. n.d., not detected. (C) Western blot of islets isolated from chow-fed WT, KO, and R138X mice. KO islets were used as negative control. The arrow indicates SLC30A8 protein; asterisks denote unspecific bands. (D) Dithizone staining of pancreatic islets isolated from WT, KO, and R138X mice.