Table 1. Antibacterial properties (IC₅₀) of 3 and [3 + 2a, c] bio-conjugates determined after incubation in serum for pre-defined times (and removal of serum proteins). Mean ± SD (n = 3–4).

Serum exposure a	IC50 (μM)
	Control (6 h, no serum)	1 h	6 h	24 h
3	8 ± 5 b	40 ± 30 d	n.a. c	n.a.
[3 + 2a]	12 ± 7	9 ± 5	8 ± 4	8 ± 5
[3 + 2c]	40 ± 20 d	20 ± 10	20 ± 10	50 ± 20 d

^aIncubation was performed under conditions that favour the forward PEGylation reaction.

^bFresh solution (0 h).

^cn.a.: No antibacterial effect was observed.

^dStatistically significant difference (ANOVA, Tukey, p = 0.05) vs. control 3 (not exposed to serum).