Fig. 6. Screening and identification of two apoptosis-independent ‘hits’. (a) Summary of the difference in cell viabilities (%) between apoptosis-resistant TC7 and apoptosis-sensitive HCT116 treated with the corresponding RAS complexes (25 μM). A smaller difference indicated a greater ability to overcome apoptosis-resistance. 22 complexes (highlighted in blue) were selected for a secondary screening. (b) Summary of the resistance factors^# (RF) of the 22 RAS complexes. A lower RF indicated a greater ability to overcome apoptosis resistance. (c) Cell viability curves of TC7 and HCT116 cells treated with various concentration of ‘hit’ compounds 532m and 532p. (d) Structures of the 2 ’hit’ compounds identified. ^#RF was calculated by taking the ratio of the eIC₅₀ in TC7 and HCT116. The eIC₅₀ was calculated based on the cell viability curves obtained from one experiment and three replicates for each concentration of drug treatment.