Fig. 7. Complexes 532m and 532p induce p53-independent, non-apoptotic cell death. (a) IC₅₀ values of 532m, 532p, oxaliplatin and 5-fluorouracil after 48 h treatment in apoptosis-sensitive (HCT116) and apoptosis-resistant (TC7) cell lines. Data represent mean ± S.E.M. of three independent experiments (*p < 0.05; two-tailed Student's t-test). (b) IC₅₀ values of 532m, 532p and oxaliplatin after 48 h treatment in the absence and presence of apoptosis inhibitor, z-VAD-fmk (40 μM). Data represent mean ± S.E.M. of three independent experiments (*p < 0.05; two-tailed Student's t-test). (c) Western blot analysis of proteins related to the apoptosis pathway and in HCT116 cells after treatment with 532m, 532p, oxaliplatin and 5-fluorouracil at 0.5× and 1× [IC₅₀] for 24 h and 48 h. Homogeneous protein loading determined with reference to actin.