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. 2018 Jun 15;26(8):2060–2069. doi: 10.1016/j.ymthe.2018.05.017

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© 2018 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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IAV Induces Apoptosis and EMAPII Release in Pulmonary Endothelium and Epithelium

(A–C and E) Human pulmonary artery endothelial cells (HPAEC), normal human bronchial epithelial cells (NHBEC), human lung microvascular endothelial cells (HLMVEC), and human alveolar epithelial line A549 were stimulated with 1 pfu/cell IAV and then analyzed for (A and B) caspase 3 cleavage, caspase 3 (A) and EMAPII (B) expression, (C) surface annexin V staining, (D) released EMAPII levels, (E) surface EMAPII staining, or (F) concomitant caspase 3 cleavage and surface EMAPII staining. *p < 0.05 by t test when compared to control values. n = 3–6, data are shown as mean ± SEM.