Figure 2.

Characterization of ASCs Harvested from Different Donors

(A) Representative micrographs of ASCs from C57BL/6, T2D, and db/db mice observed under light microscopy. (B) Representative micrographs of ASC-derived adipocytes identified by oil red O staining, osteocytes by alizarin red staining, and chondrocytes by toluidine blue staining. Scale bars, 100 μm. (C) Expression of CD105, CD29, CD45, and CD34 in ASCs analyzed by flow cytometry. Red lines represent cells stained with corresponding isotype control antibodies, and blue lines represent cells stained with individual antibodies. (D) Growth curves of C57BL/6, T2D, and db/db ASCs. (E–G) Amounts of HGF (E), TGF-β (F), and VEGF (G) secreted by different ASCs. Cells at passage three were used for the experiments. Data are from at least three individual experiments. **p < 0.01, ANOVA test. Error bars represent SD.