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Factors and testing levels for Plackett-Burman experiment for deglycosylation of genistin by the commercial β-glucosidase enzyme.
| Factor | Symbol | Low level (-1) | High level (+1) |
|---|---|---|---|
| Ph | X1 | 4 | 7 |
| Temp. (°C) | X2 | 40 | 60 |
| Time (h) | X3 | 0.5 | 3 |
| Substrate conc. (w/v) | X4 | 0.05 | 0.1 |
| Enzyme conc. (IU/mL) | X5 | 0.2 | 0.5 |
| Agitation (rpm) | X6 | 100 | 200 |
| Buffer strength (M) | X7 | 0.05 | 0.1 |
| CaCl2 (mM) | X8 | 0.5 | 1 |
| MnCl2 (mM) | X9 | 0.5 | 1 |
