Table 2.
Randomized Plakett-Burman experimental design for evaluating factors influencing deglycosylation of genistin by the commercial β-glucosidase containing enzyme.
| pH |
Temp.
(°C) |
Time (h) | Substrate conc. (mg/mL) | Enzyme conc. (IU) | Agitation (rpm) | Buffer strength (M) | CaCl 2 mM | MnCl 2 mM |
Genistein conc.
mg/g |
|
|---|---|---|---|---|---|---|---|---|---|---|
| 1 | 7 | 40 | 0.5 | 1 | 0.5 | 100 | 0.05 | 1 | 0.5 | 1.245 |
| 2 | 4 | 60 | 0.5 | 1 | 0.5 | 200 | 0.05 | 0.5 | 1 | 2.574 |
| 3 | 7 | 60 | 3 | 1 | 0.2 | 100 | 0.05 | 0.5 | 1 | 1.734 |
| 4 | 4 | 40 | 0.5 | 0.5 | 0.2 | 100 | 0.1 | 0.5 | 1 | 1.1 |
| 5 | 4 | 60 | 3 | 0.5 | 0.2 | 100 | 0.05 | 1 | 0.5 | 2.68 |
| 6 | 7 | 40 | 3 | 1 | 0.2 | 200 | 0.1 | 1 | 1 | 2.142 |
| 7 | 7 | 40 | 0.5 | 0.5 | 0.2 | 200 | 0.05 | 0.5 | 0.5 | 0.633 |
| 8 | 4 | 60 | 0.5 | 1 | 0.2 | 200 | 0.1 | 1 | 0.5 | 2.028 |
| 9 | 4 | 40 | 3 | 1 | 0.5 | 100 | 0.1 | 0.5 | 0.5 | 4.05 |
| 10 | 7 | 60 | 0.5 | 0.5 | 0.5 | 100 | 0.1 | 1 | 1 | 1.044 |
| 11 | 4 | 40 | 3 | 0.5 | 0.5 | 200 | 0.05 | 1 | 1 | 4.167 |
| 12 | 7 | 60 | 3 | 0.5 | 0.5 | 200 | 0.1 | 0.5 | 0.5 | 3.378 |
N.B: Starting genistein concentration is 0.8 mg/g soy flour.