Table 4.
Box-Behnken design table; (+1): high level; (–1): low level; (0) central point. The amount obtained for biomass and organic selenium is derived from experimental data.
| Trial No. |
Variables
|
Biomass (g/L) | Organic selenium (ppm) | |||
|---|---|---|---|---|---|---|
|
D
(Glucose) |
F
(Aeration) |
A
(Selenium) |
B
(Temperature) |
|||
| 1 | -1 | -1 | 0 | 0 | 1.16 | 1734 |
| 2 | 1 | -1 | 0 | 0 | 3.67 | 1632 |
| 3 | -1 | 1 | 0 | 0 | 3.64 | 1967 |
| 4 | 1 | 1 | 0 | 0 | 6.23 | 1957 |
| 5 | 0 | 0 | -1 | -1 | 3.99 | 506 |
| 6 | 0 | 0 | 1 | -1 | 4.18 | 1363 |
| 7 | 0 | 0 | -1 | 1 | 4.29 | 1705 |
| 8 | 0 | 0 | 1 | 1 | 4.27 | 2609 |
| 9 | -1 | 0 | 0 | -1 | 3.25 | 1249 |
| 10 | 1 | 0 | 0 | -1 | 4.94 | 1202 |
| 11 | -1 | 0 | 0 | 1 | 1.89 | 2429 |
| 12 | 1 | 0 | 0 | 1 | 4.59 | 2482 |
| 13 | 0 | -1 | -1 | 0 | 3.29 | 980 |
| 14 | 0 | 1 | -1 | 0 | 5.59 | 1320 |
| 15 | 0 | -1 | 1 | 0 | 2.76 | 1789 |
| 16 | 0 | 1 | 1 | 0 | 5.25 | 2147 |
| 17 | -1 | 0 | -1 | 0 | 2.99 | 1139 |
| 18 | 1 | 0 | -1 | 0 | 4.45 | 1054 |
| 19 | -1 | 0 | 1 | 0 | 2.43 | 1934 |
| 20 | 1 | 0 | 1 | 0 | 4.78 | 1987 |
| 21 | 0 | -1 | 0 | -1 | 2.80 | 1110 |
| 22 | 0 | 1 | 0 | -1 | 5.83 | 1318 |
| 23 | 0 | -1 | 0 | 1 | 2.61 | 2334 |
| 24 | 0 | 1 | 0 | 1 | 5.73 | 2535 |
| 25 | 0 | 0 | 0 | 0 | 4.18 | 1857 |
| 26 | 0 | 0 | 0 | 0 | 4.38 | 1876 |
| 27 | 0 | 0 | 0 | 0 | 4.41 | 1916 |
| 28 | 0 | 0 | 0 | 0 | 4.45 | 1934 |
| 29 | 0 | 0 | 0 | 0 | 4.54 | 1897 |