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. 2018 Summer;17(3):995–1007.

Figure 2.

Figure 2

Generation ROS in isolated human lymphocyte after treatment with PFOS. ROS generation in human lymphocyte after treatment with PFOS for different time intervals. ROS generation was measured in cells using dichlorofluoresceindiacetate (DCFH-DA) and fluorescence spectrophotometer. Induction of ROS by PFOS was significant (P < 0.05) at concentration 150 and 300 µM at 2 h and at all concentration at 4 h and at 6 h only at concentration 75 µM in comparison with control, but ROS formation after 6 h significantly(P < 0.05) increased until 12 h in comparison with control. Buyhylatedhydroxytoluene (BHT), an antioxidant, inhibited ROS induction by PFOS in human lymphocytes. *P < 0.05, **P < 0.01 and ***P < 0.001.