Table 4.

Effect of storage on change in constituent separation, size and EE% of phytoniosomes at different time intervals after preparation.

ID	Storage condition	Constituent separation			Mean volume diameter (µm) ± SD			EE% ± SD
		2 weeks	1 month	3 months	2 weeks	1 month	3 months	2 weeks	1 month	3 months
F1	I	N	N	N	6.04±0.43	6.36±0.58	7.60±0.81	46.9±2.4	57.0±2.1	58.4±3.3
	II	P	P	P	5.79±0.25	6.58±0.79	24.52±5.14	54.2±2.7	59.7±3.2	62.0±3.7
	III	P	P	P	6.40±0.30	12.11±1.35	13.68±3.21	49.0±3.5	60.9±2.2	62.3±3.1
F2	I	N	N	N	6.67±0.55	6.94±0.46	8.09±1.17	65.7±2.6	76.5±4.2	74.7±3.7
	II	N	P	P	6.20±0.30	6.57±0.74	8.02±1.08	67.4±2.1	71.9±3.9	76.1±1.0
	III	P	P	P	6.67±0.56	8.43±0.98	69.92±9.94	67.3±3.1	79.7±4.1	82.6±4.3
F4	I	N	N	N	7.19±0.86	7.97±1.72	8.00±0.79	74.8±2.2	80.4±2.0	86.1±3.4
	II	N	P	P	6.98±0.52	7.94±0.57	8.64±1.12	87.7±3.1	88.5±2.9	83.8±4.4
	III	P	P	P	8.48±1.48	9.79±2.04	34.39±12.96	65.5±2.9	66.8±2.3	76.1±4.9
F5	I	N	N	N	7.23±0.24	7.59±0.56	7.77±0.46	92.2±3.4	95.0±2.3	95.8±4.4
	II	N	P	P	7.20±0.46	7.68±0.36	8.89±0.71	88.7±3.7	91.2±2.9	92.3±2.7
	III	N	P	P	7.62±0.25	8.72±0.64	9.85±0.98	87.7±2.5	87.9±3.5	91.1±3.4
F6	I	N	N	N	8.96±2.46	8.50±0.62	9.77±1.69	95.1±3.2	97.6±3.1	97.8±4.2
	II	N	P	P	7.98±0.96	8.90±1.24	9.66±1.09	94.4±4.2	92.6±5.3	93.4±4.7
	III	P	P	P	7.50±0.62	9.80±1.48	13.67±2.88	93.5±3.1	93.9±3.8	97.1±4.2

EE% entrapment efficiency percent.

F1; S40: T40: Chol (3.5:3.5:3 molar ratio)

F2; S40: T40: Chol (3:3:4 molar ratio)

F4; S60: T60: Chol (3.5:3.5:3 molar ratio)

F5; S60: T60: Chol (3:3:4 molar ratio)

F6; S60: T60: Chol (2.5:2.5:5 molar ratio)

I; 4 °C, II; 25 °C , 30% RH, III; 40 °C, 70% RH

N; normal, P; precipitate