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. 2018 Jun 15;26(8):2047–2059. doi: 10.1016/j.ymthe.2018.05.019

Figure 1.

Figure 1

Identification and In Vitro Pharmacodynamic Characterization of Aptamers with Higher hTLR4 Binding Affinity

(A) Graph showing binding of the selected aptamers to hTLR4 in ELONA assays. Data represent mean ± SEM of three independent experiments performed in triplicate. (B) Fold increase aptamer recovery after incubation of resin-hTLR4-protein complexes with ApTLR#1R and ApTLR#4F relative to RND40 (n = 3) by qPCR. (C) Fold increase aptamer recovery after incubation of 293-hTLR4A cells with ApTLR#1R and ApTLR#4F relative to HEK293 (n = 3) by qPCR. (D) SEAP assay for the characterization of the antagonistic effect of ApTLR#1R and ApTLR#4F on hTLR4. HB-hTLR4 cells were exposed to LPS (0.1 ng/mL; left) or DAMPs (5 μL; right) 1 hr prior to the addition of aptamers (0.2–200 nM) to the medium. Data are expressed as the percentage of SEAP activity relative to LPS or DAMPs alone (agonist control) (n = 9). Student’s t test (*p < 0.05 versus agonist control).