Figure 1.

MGC Aggregation Is Directly Proportional to Disease Severity of Preclinical Models

(A) Live OCT images were taken from three mutant mouse models (Pde6b^H620Q/H620Q, Pde6b^STOP/STOP, and Pde6a^D670G/D670G) at 4 weeks as compared to WT, with the red bar indicating the ONL and IS/OS layers (scale bar, 100 μm). (B) Live SLO images were taken from WT and three mutant mouse models at 4 weeks. The white dots, obtained by cSLO, are GFP expressed under the control of the Cx3cr1 promoter in MGCs (scale bar, 1 mm). (C) Photoreceptor thickness was measured and quantified based on OCT images from WT and mutant mice. p < 0.001 for WT compared to all three mutant groups; p < 0.001 for Pde6a^D670G/D670G compared to Pde6b^H620Q/H620Q and Pde6b^STOP/STOP. For WT, n = 10 eyes. For all mutant groups, n = 8 eyes. (D) The MGC density was captured using SLO at 4 weeks and measured and quantified by ImageJ in WT and mutant mice. p = 0.004 between WT and Pde6b^H620Q/H620Q; p < 0.001 for WT compared to Pde6b^STOP/STOP and Pde6a^D670G/D670G. p = 0.002 between Pde6b^H620Q/H620Q and Pde6b^STOP/STOP; p = 0.02 between Pde6b^H620Q/H620Q and Pde6a^D670G/D670G; p < 0.001 between Pde6b^STOP/STOP and Pde6a^D670G/D670G. For WT, n = 10 eyes. For all mutant groups, n = 8 eyes. OCT, optical coherence tomography; ONL, outer nuclear layer; IS/OS, inner and outer segment layers; cSLO, confocal scanning laser ophthalmoscope. *p < 0.05; **p < 0.01; ***p < 0.001. Error bars are 1 SD.