Figure 3.

MGC Density Increases in Conjunction with Increased Photoreceptor Degeneration

(A and B) Live OCT (A) and SLO (B) images were taken from the Pde6b^H620Q/STOP;Pde6g^Cre/+ mouse model at 4, 6, 8, 12, and 16 weeks, while the WT was imaged at 4 weeks (red bar, ONL and IS/OS; for OCT, scale bar, 100 μm; for SLO, scale bar, 1 mm). (C and D) Photoreceptor thickness and MGC density were measured and quantified using the techniques outlined in Figure 1. (C) p < 0.001 for WT compared to each mutant mouse and between each mutant mouse. (D) p < 0.001 for WT compared to mutant mice at all time points. No statistically significant difference was found between 6, 8, and 12 weeks in the mutant mouse. p = 0.002 between 4 and 6 weeks. (E and F) Retinal cross sections (E) and retinal flat mounts (F) were stained at 4, 6, 8, 12, and 16 weeks for the mutant mice compared to at 4 weeks for WT (blue, DAPI; green, GFP; yellow bar, ONL; scale bar, 25 μm). *p < 0.05; **p < 0.01; ***p < 0.001. Error bars are 1 SD.