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. 2018 Aug 16;11:225. doi: 10.1186/s13068-018-1224-7

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© The Author(s) 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Enzymatic saccharification efficiency of transgenic events and WT. Glucan conversion rates of untreated biomass (A), heat-treated biomass (B), and heat-treated biomass followed by a mild acid pretreatment (C). Liquid hydrolysate was collected at different timepoints over 72–120 h, and glucose measured by ion-exchange chromatography. D Comparison of glucan conversion rates at 72 h of untreated biomass, heat-treated biomass, and pretreated biomass of transgenic events and WT. E Comparison of released glucose concentration of CaMV-4 and WT among three treatments. Each bar represents mean of three individual plants per event with the standard error. Different letters represent significant differences among the samples (p ≤ 0.05) as calculated by one-way ANOVA followed by Tukey post hoc analysis