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. 2018 Jul 26;9:999. doi: 10.3389/fphys.2018.00999

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Copyright © 2018 Sun, Cheung, Au, Yeung and Yeung.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Specific overexpression of MGF in pMGF-electroporated muscles. The expression of (A) Mgf and (B) Igf-1Ea in pMGF-electroporated muscles harvested from 1 to 8 days post-electroporation (n = 5/treatment/timepoint). This is relative to 8 days vector. Significant difference between vector and pMGF: ^∗∗∗P < 0.001. The expression of (C) Mgf and (D) Igf-1Ea in pMGF-electroporated CTX-injured muscles harvested from 2 to 7 days post-injury (n = 6/treatment/timepoint). This is relative to 7 days vector + CTX. Significant difference between vector + CTX and pMGF + CTX: ^∗∗∗P < 0.001. The geometric mean of Gapdh, 18S rRNA, and Rsp20 expression served as the internal control. Statistics were analyzed by two-way ANOVA and followed by Bonferroni test. Values represent mean ± SEM.