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. 2018 Jul 26;9:999. doi: 10.3389/fphys.2018.00999

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Copyright © 2018 Sun, Cheung, Au, Yeung and Yeung.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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No observable change in inflammatory cytokine expression in MGF-overexpressing C2C12 myogenic cells in vitro. The expression of (A) Mgf, (B) Tnf-α, and (C) Ccl2 in myoblast and myotube cultures harvested at 24 and 48 h post-transfection (n = 3/cell/treatment/timepoint). Gapdh expression served as the internal control. The expression level is relative to 48 h vector control. Significant difference between vector and pMGF: ^∗P < 0.05, ^∗∗P < 0.01, and ^∗∗∗P < 0.001. Statistics were analyzed by two-way ANOVA and followed by Bonferroni test. Values represent mean ± SEM.