Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Jul 25;9:1725. doi: 10.3389/fimmu.2018.01725

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2018 Moonen, Alders, Bontkes, Schoenmaker, Nicu, Loos and de Vries.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Gating tree of flow cytometry experiments. The antibody combination for flow cytometric experiments was CD14-V450/CD19-APC/CD3-V500/CD56-PE/CD4-PerCPCy5.5/CD8-APC-Cy7/CD196-PE-Cy7 (Table 1). Acquisition and analysis were performed on a BD FACSverse flow cytometer using associated FACSuite software. The number in quadrants indicate percentages of cells in the corresponding gating. The gating tree was set as follows: (A) forward scatter/sideward scatter represents the distribution of cells in the light scatter based on size and intracellular composition, respectively, where the live population is encircled in purple. In the “live” population, (B) CD14+, (C) CD19+, CD3+, (D) CD56+CD3− cells were identified. In the CD3+ gating, (E) CD4+, CD8+, and (F) CD196+ populations were identified. Here, a characterization of a gingival fibroblast-peripheral blood mononuclear cell coculture after 21 days is shown.