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. 2018 Jul 25;9:1725. doi: 10.3389/fimmu.2018.01725

Figure 4.

Figure 4

Peripheral blood lymphocytes cells survive in presence of gingival fibroblasts (GFs) after 21 days. Distribution of CD56+CD3−, CD3+, CD14+, and CD19+ as indicated by stack bars over time; (A) before coculturing (0 days), (B) after 7 days, and (C) after 21 days. The proportion of cells (in percentages ± SEM) in live populations of (A) monocultures and (B,C) cocultures are presented in the table below (A–C) per cell subtype for cocultures with GFs. (D–G) Specification of (C) at 21 days. (D) CD3+ T cells, (E) CD19+ B cells, (F) CD56+CD3− NK cells, (G) CD14+ monocytes. (D–F) For all peripheral blood lymphocytes (PBLs), significantly more events per microliters were detected in cocultures with peripheral blood mononuclear cells and PBLs. (H) Specification of CD3+ cell population at baseline levels where CD3+CD4+, CD3+CD8+, and CD3+CD4+CD196+ T cells are shown. (I) Specification of CD3+ cell populations after 21 days where CD3+CD4+, CD3+CD8+, and CD3+CD4+CD196+ T cells are shown. Tables below figures (H,I) represent proportion (percentages ± SEM) of cell populations per (H) monoculture and (I) coculture. Data are presented as events per microliter (A–C,H,I) ±SEM (D–G). n = 10 GF donors, n = 2 buffy coats, *p < 0.05, **<0.01.