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. 2018 Jul 25;9:1725. doi: 10.3389/fimmu.2018.01725

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Copyright © 2018 Moonen, Alders, Bontkes, Schoenmaker, Nicu, Loos and de Vries.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Survival and retention of peripheral blood lymphocytes in coculture of gingival fibroblast (GF) over time. Percentages of cells present in cocultures. (A) The proportion of CD14+ cells (percentage ± SEM) present in GF cocultures with peripheral blood mononuclear cells (PBMCs) (solid line) and monocytes (dotted line). (B) Geometric mean fluorescence intensity (gMFI) of CD14+ cells over time of GF cocultures with PBMCs (solid line) and monocytes (dotted line). No significant difference of CD14+ percentage (A) or gMFI (B) after 7 days was observed. Percentages ±SEM of (C) CD19+, (D) CD56+CD3−, (E) CD3+, (F) CD3+CD4+, (G) CD3+CD8+, (H) CD3+CD4+CD196+ cells present in GF cocultures with PBMCs (solid line) and peripheral blood lymphocytes (PBLs) (dotted line). No significant increase or decrease of percentage (D) CD56+CD3−, (E) CD3+, (F) CD3+CD4+ cells is shown over time in both cocultures of PBMCs and PBLs. (C) A significant decrease of CD19+ cells after 7 days is shown in PBL cocultures. Significant decrease of CD3+CD8+ cells is shown after 7 days in PBMC cocultures. A significant decrease of CD3+CD4+CD196+ cells after 14 days is shown in PBMC and PBL cocultures. Time point 0 represents monocultures of (A,B) monocytes, (C–H) PBMCs, and PBLs. All significant differences were tested after 7 days since time point 0 represents a characterization of one experiment. n = 10 GF donors, n = 2 buffy coats, *p < 0.05, **<0.01.