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. 2018 Jul 25;9:1725. doi: 10.3389/fimmu.2018.01725

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Copyright © 2018 Moonen, Alders, Bontkes, Schoenmaker, Nicu, Loos and de Vries.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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CD3+CD4+ and CD3+CD8+ cells proliferate in the presence of gingival fibroblasts (GFs). Quantification of proliferating Carboxyfluorescein succinimidyl ester (CFSE)-labeled CD3+ cells over time. (A) Dot plots of CD3+ cells within peripheral blood mononuclear cell (PBMC) (left panels) and peripheral blood lymphocyte (PBL) (right panels) cocultures after 7 (upper panels) and 14 days (lower panels). CFSE intensity is plotted on the y-axis and CD8+ is plotted on the x-axis. CD3+CD8+ cells are shown in red, CD3+CD8− (CD3+CD4+) cells are shown in green. Numbers in lower quadrants indicate the percentages of cells that have divided. (B) Quantification of the divided CD4+ (white bars) and CD8+ (gray bars) cells (normalized percentages ± SEM) in PBMC cocultures over time. (C) Quantification of the divided CD4+ (white bars) and CD8+ (gray bars) cells (normalized percentages ± SEM) in PBL cocultures over time. All significant differences were compared to 7 days. n = 5 GF donors, *p < 0.05, **<0.01, ***<0.001.