Figure 2.

Heterologous expression of Kv1.1-WT and G311D mutant channels in Xenopus oocytes. (A) Averaged current amplitudes computed at +60 mV for Kv1.1-WT, G311D, and WT+G311D channels at 24, 48, and 72 h post cRNA injections. Each data point is the mean±SE of 8 cells.(B) Bar graph current amplitudes recorded 48 h after injection. The mutant channel shows ~3-fold smaller current than the WT (*p < 0.05, **p < 0.005, Student t-test). (C) Representative Kv1.1 current traces recorded at +60 mV from a holding potential of−80 mV showing reduction in oocytes expressing G311D cRNA.