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. 2018 Aug 9;5:216. doi: 10.3389/fmed.2018.00216

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Copyright © 2018 Iansante, Dhawan, Masmoudi, Lee, Fernandez-Dacosta, Walker, Fitzpatrick, Mitry and Filippi.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Viability, albumin and urea production of hepatocytes (HC) encapsulated in alginate microdisks with and without mesenchymal stromal cells (MSC). (A) Cell viability measured by calcein-AM assay and expressed as % over baseline value at day 0. Cells were released by incubation of microdisks with EDTA. (B) Albumin synthesis measured by ELISA on cell supernatants. (C) Urea production measured using a colorimetric assay on cell supernatant following incubation with 5 mM ammonium chloride for 6 h. Ureogenesis is expressed as μg of urea released in 1 h by 10⁶ cells. N = 3. Data represent mean ± SEM. *p < 0.05; ****p ≤ 0.0001.