Figure 5.
Viability, albumin and urea production of hepatocytes (HC) encapsulated in alginate microbeads with and without mesenchymal stromal cells (MSC). (A) Cell viability measured by calcein-AM assay and expressed as % over baseline value at day 0, after release from the alginate. (B) Albumin synthesis measured by ELISA on cell supernatants. (C) Urea production measured using a colorimetric assay on cell supernatant following incubation with 5 mM ammonium chloride for 6 h. Ureogenesis is expressed as μg of urea released in 1 h by 106 cells. N = 3. Data represent mean ± SEM. *p < 0.05; **p < 0.01; ****p ≤ 0.0001.