Table 1.

List of the oligonucleotides used in this study.

Sequence ID∗	5′→3′ sequence	Product length (bp)	GC (%)
Ptr–Forward	GTCTCCTCTGGTGGTATG	99	55.6
Ptr–Reverse	GCTCTTAGTGAAGTTCAATC
Ptr–Probe	TACCTCTACTCGGTCGCCTATGG
Pn–Forward	ACCGCATTACCAAAGTTC	112	45.8
Pn–Reverse	ACTGGAACTGGAACAATAAG
Pn–Probe	CCTGAATGCTCTTGACACTTGGTT

^∗Ptr and Pn refer to P. tritici-repentis and Pa. nodorum, respectively. Sequences in bold are pre-published primers modified from Oliver et al. (2008). Other sequences are designed in this study. Primers and probes were designed to amplify short fragments of the targeted pathogen DNA.