Fig. 2.

VGLUT-positive inspiratory interneurons in the third thoracic cord. A,B: A VGLUT-positive inspiratory interneuron. Aa, Ba, recordings obtained from the fourth cervical and third thoracic ventral root (C4, T3) and the membrane potential of the inspiratory interneuron (MP). Ab and Bb are fluorescence images. The recorded neuron was stained by Lucifer yellow (green). Ac and Bc are higher magnifications of the highlighted square in panels Ab and Bb. The VGLUT-positive cells were stained with black. Ad, Ae, Bd, Be: Bright-field images of the same areas as Ab, Ac, Bb, Bc, respectively. White and black arrows indicate the recorded neuron.