VGLUT-negative inspiratory interneuron in the third thoracic cord. A: Recordings obtained from C4 and T3 thoracic ventral root, and membrane potential of the inspiratory interneuron. Ba, b: Fluorescence images. Bc, d: Bright-field images of the same areas as Ba and b. Bb, d: Higher magnifications of the highlighted square in panels Ba, c. White and black arrows indicate the recorded neuron. Since the VGLUT-positive cells were stained with black, the recorded neuron was negative.