Table 2.
Changes of AP parameters in subepicardial (subEpi) and subendocardial (subEndo) cardiomyocytes under the effects of FCCP (n = 9 for each).
| dV/dtmax (V/s) | RP (mV) | APA (mV) | APD20 (ms) | APD50 (ms) | APD90 (ms) | |
|---|---|---|---|---|---|---|
| subEpi | ||||||
| Control | 104.8 ± 6.7# | –76.1 ± 0.5# | 104.3 ± 1.5# | 85.3 ± 2.7 | 128.1 ± 2.4# | 157.2 ± 2.1# |
| FCCP↑ | 69.0 ± 5.2*,# | –70.4 ± 1.4*,# | 89.5 ± 5.2* | 87.6 ± 2.8* | 137.3 ± 2.9* | 173.9 ± 3.4* |
| FCCP 2.5′ | 48.7 ± 5.4*,# | –67.5 ± 1.8* | 87.9 ± 3.3* | 58.9 ± 5.5* | 92.4 ± 8.2* | 122.9 ± 9.5* |
| FCCP 5′ | 49.1 ± 7.3*,# | –67.9 ± 1.4* | 71.2 ± 7.9* | 23.4 ± 5.7* | 36.7 ± 8.3* | 60.8 ± 9.4* |
| subEndo | ||||||
| Control | 148.5 ± 15.8 | –79.1 ± 0.3 | 109.8 ± 1.5 | 87.6 ± 3.1 | 133.3 ± 2.3 | 161.3 ± 2.2 |
| FCCP↑ | 107.7 ± 10.8* | –74.8 ± 0.9* | 98.0 ± 3.9* | 93.2 ± 2.8* | 147.3 ± 3.5* | 182.1 ± 3.8* |
| FCCP 2.5′ | 82.1 ± 9.7* | –70.9 ± 1.4* | 89.3 ± 5.6* | 62.8 ± 3.9* | 98.1 ± 6.9* | 127.4 ± 8.4* |
| FCCP 5′ | 92.0 ± 14.7* | –70.0 ± 2.4* | 78.4 ± 9.4* | 35.8 ± 6.1* | 56.9 ± 8.5* | 86.5 ± 7.7* |
Note that the depths of microelectrode insertion for subEpi and subEndo recordings were 0.59 ± 0.12 and 2.96 ± 0.18 mm from the epicardium, respectively. Other notations are the same as in Table 1. ∗p < 0.05 vs. control. #p < 0.05 subEpi vs. subEndo values obtained at control and at the same time of FCCP action.