Table 1.
Primers for PCR
| Gene | Sequence | Amplicon (bp) |
|---|---|---|
| GAPDH | F‐GAGTCAACGGATTTGGTCGT | 238 |
| R‐TTGATTTTGGAGGGATCTCG | ||
| RUNX2 | F‐CCTCGGAGAGGTACCAGATG | 247 |
| R‐TTCCCGAGGTCCATCTACTG | ||
| OSX (SP7) | F‐GCCAGAAGCTGTGAAACCTC | 161 |
| R‐GCTGCAAGCTCTCCATAACC | ||
| ALP | F‐CCTTGCTCACTCACTCACTCC | 182 |
| R‐TTTTTTTTGCCGTTCCAAAC | ||
| COL1A1 | F‐AGGGCCAAGACGAAGACATCCC | 108 |
| R‐TGTCGCAGACGCAGATCCG | ||
| OCN (BGLAP) | F‐GTGCAGAGTCCAGCAAAGGT | 152 |
| R‐TCAGCCAACTCGTCACAGTC | ||
| OPG | F‐AACGGCAACACAGCTCACAAGAAC | 160 |
| R‐TGCTCGAAGGTGAGGTTAGCATGT | ||
| RANKL | F‐ATCGTTGGATCACAGCACATC | 152 |
| R‐AGACTCACTTTATGGGAACCAGA | ||
| SPHK1 | F‐AGGCTGAAATCTCCTTCACGC | 113 |
| R‐GTCTCCAGACATGACCACCAG | ||
| S1PR1 | F‐ATATCAGCGCGGACAAGGAG | 136 |
| R‐CACTTGCAGCAGGACATGATCC | ||
| S1PR2 | F‐TTCGTGCGACTACAGAACCACTGT | 113 |
| R‐AGAAGTCGTCAAGTGGCAGCTGAT | ||
| S1PR3 | F‐CGGCATCGCTTACAAGGTCAA | 99 |
| R‐GCCACGAACATACTGCCCT | ||
| S1PR4 | F‐GACGCTGGGTCTACTATTGCC | 135 |
| R‐CCTCCCGTAGGAACCACTG | ||
| S1PR5 | F‐GCGCACCTGTCCTGTACTC | 94 |
| R‐GTTGGTGAGCGTGTAGATGATG | ||
| SGPP1 | F‐CTGGTGTTCTCTAGTTTGCCTAAG | 136 |
| R‐GGTTGAAGTTGTCAATCAGGTCC | ||
| ENPP1 | F‐TTGGACCCTCAGTGGCAACTTG | 136 |