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. 2018 May 31;96(8):3196–3207. doi: 10.1093/jas/sky220

Table 4.

Characterization of skeletal muscle metabolic profile as determined in the LM from low (LRFI) and high (HRFI) residual feed intake pigs in control and Mycoplasma hyopneumoniae + Lawsonia intracellularis dual-challenged (MhLI) groups at days post inoculation (dpi) 21

Item Treatment SEM P-value
LRFI-control HRFI-control LRFI-MHLI HRFI-MHLI MHLI Line MHLI x Line
µ-Calpain1 2.85a 4.19bc 3.17ab 4.20c 0.314 0.551 0.012 0.592
m-Calpain1 7.59 7.19 8.00 6.76 0.499 0.984 0.240 0.362
Calpastatin I1 3.34a 2.50a 4.21b 3.63b 0.530 0.016 0.387 0.739
Calpastatin II1 7.28 5.94 6.46 5.51 0.978 0.460 0.395 0.832
Total calpastatin1 10.69 8.36 10.72 9.10 1.256 0.722 0.260 0.757
µ-Calpain:calpastatin2 0.27a 0.51ab 0.31ab 0.48b 0.064 0.849 0.033 0.600
20s proteasome1 2.32 12.80 2.45 7.24 4.796 0.368 0.309 0.394
ERMs3,4 0.199 0.222 0.234 0.251 0.064 0.461 0.813 0.948
ROS
 Glutamate5 0.16 0.14 0.25 0.34 0.096 0.088 0.797 0.533
 Succinate6 0.11 0.12 0.14 0.13 0.022 0.392 0.945 0.664
Protein carbonyls7 0.32 0.44 0.25 0.36 0.090 0.296 0.366 0.916
Hexokinase8 468 963 683 523 253.2 0.558 0.603 0.129
Citrate Synthase9 2003 2412 2029 1791 387.9 0.236 0.868 0.244
CS:HK, AU 4.84a 6.06ab 0.07bc −1.07c 3.081 0.018 0.985 0.217
Protein:RNA 225 371 275 297 40.14 0.722 0.091 0.089

Estimates are represented by 6 pigs per line x challenge.

a,b,cMeans with differing superscripts indicate a significant (P < 0.05) difference.

1Units of activity/g protein.

2Ratio of µ-calpain activity to total calpastatin activity.

3ERMs = easily releasable myofilaments.

4Percent ERMS/crude myofibrillar extract.

5Basal reactive oxygen species (ROS) production from isolated mitochondria as H202 produced, μmol/min/mg mitochondrial protein, when glutamate is provided as a substrate.

6Basal ROS production from isolated mitochondria as H202 produced, μmol/min/mg mitochondrial protein, when succinate is provided as a substrate.

7nmol protein carbonyls/mg sarcoplasmic protein.

8Hexokinase (HK) activity reported as μmol/min/mg sarcoplasmic protein.

9Citrate Synthase (CS) activity reported as μmol/min/mg sarcoplasmic protein.