Table 5.

Activity of key liver gluconeogenic enzymes and mitochondrial reactive oxygen species (ROS) production from low (LRFI) and high (HRFI) residual feed intake pigs in control and Mycoplasma hyopneumoniae + Lawsonia intracellularis dual-challenged (MhLI) groups at days post inoculation (dpi) 21

Item	Treatment				SEM	P-value
	LRFI-control	HRFI-control	LRFI-MHLI	HRFI-MHLI		MHLI	Line	MHLI x Line
Gluconeogenesis
G6P1	0.18	0.24	0.18	0.21	0.027	0.408	0.252	0.412
F16BP2	1.91a	2.05a	1.21b	1.62ab	0.214	0.037	0.326	0.371
PEPCK3	11.24	10.26	8.83	12.29	2.271	0.914	0.487	0.080
ROS
Glutamate4	0.46a	0.59ab	0.73b	0.70ab	0.125	0.034	0.800	0.339
Succinate5	0.65 a	0.88 ab	1.09b	0.99b	0.087	0.002	0.552	0.053
Protein:RNA	58.0	64.4	50.9	61.5	5.974	0.321	0.239	0.684

Estimates are represented by 6 pigs per line x challenge.

^a,b,cMeans with differing superscripts indicate a significant (P < 0.05) difference.

¹Pi released, mM/min/mg protein.

²NADPH produced, μmol/min/mg protein.

³ATP produced, μM/min/μg protein.

⁴Basal ROS production from isolated mitochondria as H₂0₂ produced, μmol/min/mg mitochondrial protein when glutamate is provided as a substrate.

⁵Basal ROS production from isolated mitochondria as H₂0₂ produced, μmol/min/mg mitochondrial protein when succinate is provided as a substrate.