Table 1.

Diet composition and analyzed nutrient content of complete diet containing steeped DDGS¹

Item	Control2	FDE-A3	FDE-B4
Ingredient, % as fed
Steeped corn DDGS	65.0	65.0	65.0
Corn starch	28.9	28.9	28.9
Sucrose	2.06	2.06	2.06
Cellulose	0.69	0.69	0.69
Corn oil	0.69	0.69	0.69
Limestone	1.35	1.35	1.35
Mono-calcium phosphate	0.20	0.20	0.20
Salt	0.30	0.30	0.30
Vitamin and mineral premix5	0.60	0.60	0.60
Titanium dioxide	0.20	0.20	0.20
Analyzed
DM, % DM	91.0	90.9	91.2
CP, % DM	18.7	18.5	17.1
Ash, % DM	6.17	6.41	6.10
Crude fat, % DM	6.42	6.89	6.23
NDF, % DM	24.2	22.5	23.1
ADF, % DM	7.10	6.86	7.07
GE, kcal/kg	4,434	4,411	4,377
Calcium, % DM	0.51	0.51	0.51
Phosphorus, % DM	0.60	0.60	0.60
Sodium, % DM	0.34	0.34	0.34

DDGS, distillers dried grains with solubles; FDE, fiber-degrading enzymes.

¹Steeped (350 g of DDGS and 1.5 liters of water) for 24 h at 40 °C with 15 min of agitation every 40 min. Mixed with other feedstuffs prior to feeding.

²No enzymes.

³Supplied 5,500 U of xylanase and 1,050 U of β-glucanase per kg of DDGS.

⁴Supplied 1,200 U of xylanase, 150 U of β-glucanase, 500 U of cellulase, and 5,000 U of protease per kg of DDGS.

⁵Provided per kilogram of diet: vitamin A, 12,000 IU as retinyl acetate; vitamin D3, 1,200 IU as cholecalciferol; vitamin E, 48 IU as dl-α-tocopherol acetate; vitamin K, 3 mg as menadione; pantothenic acid, 18 mg; riboflavin, 6 mg; choline, 600 mg; folic acid, 2.4 mg; niacin, 30 mg; thiamine, 18 mg; pyridoxine, 1.8 mg; vitamin B12, 0.03 mg; biotin, 0.24 mg; Cu, 18 mg from CuSO₄ × 5H₂O; Fe, 120 mg from FeSO₄; Mn, 24 mg from MnSO₄; Zn, 126 mg from ZnO; Se, 0.36 mg from Na₂SeO₃; and I, 0.6 mg from KI (DSM Nutritional Products Canada Inc., Ayr, ON, Canada).