Table 3.

Oligonucleotide primers and probes used for bacteriological analysis

Primers/probes		Sequence (5′–3′)	Tm (°C)	Product size (bp)
Escherichia coli	Forward	CATGCCGCGTGTATGAAGAA	57	96
	Reverse	CGGGTAACGTCAATGAGCAAA
	Probe	AGGTATTAACTTTACTCCCTTCCTC
Lactobacilli	Forward	GAGGCAGCAGTAGGGAATCTTC	55.7	126
	Reverse	CAACAGTTACTCTGACACCCGTTCTTC
	Probe	AAGAAGGGTTTCGGCTCGTAAAACTCTGTT
Total bacteria	Forward	ACTCCTACGGGAGGCAGCAG	64.5	200
	Reverse	ATTACCGCGGCTGCTGG