Table 1.

Ingredient composition of depletion diets (CON¹ or ANT²) fed throughout the trial (DM basis, %)

	Depletion Diet
	CON	ANT
Ingredient
Corn silage	40	40
Dry rolled corn	25	25
DDGS3	20	15
MDGS4	10	10
S and Mo premix5	0	5
Micro ingredients6	5	5
Calculated composition7
CP, %	15.06	14.90
NDF, %	26.03	25.86
EE, %	5.16	5.11
Analyzed composition8
S, % of DM	0.22	0.48
Cu, mg/kg DM	6.98	2.61
Mn, mg/kg DM	17.53	7.52
Mo, mg/kg DM	0.39	2.03
Zn, mg/kg DM	29.55	14.72

¹Control diet provided supplemental trace minerals per kg diet DM: 10 mg of Cu (CuSO₄), 30 mg of Zn (ZnSO₄), 20 mg of Mn (MnSO₄), 0.5 mg of I (ethylenediamine dihydroiodide), 0.1 mg of Se (sodium selenite), and 0.1 mg of Co (cobalt carbonate).

²Antagonist diet provided supplemental trace minerals per kg diet DM: 0.5 mg of I (ethylenediamine dihydoiodide) and 0.1 mg of Co (cobalt carbonate).

³Dried distillers grains with solubles.

⁴Modified distillers grains with solubles.

⁵S and Mo premix use DDGS as a carrier and provide 0.3% S (as CaSO₄) and 2 mg of Mo/kg DM.

⁶Microingredients include trace minerals, Vitamin A, limestone, salt, and Rumensin, and use DDGS as a carrier.

⁷Calculated based on individual ingredient analysis from Dairyland Laboratories (Arcadia, WI). CP, crude protein; NDF, neutral detergent fiber; EE, ether extract.

⁸Analysis of TMR from depletion period.