Table 2.
Composition of experimental treatments of the Rusitec DAIRY (50:50 roughage: concentrate) and BEEF (15:85 roughage: concentrate) trials1
| Item, DM, % (unless otherwise stated) | 0 EN | 1.25 EN | 2.5 EN | |||
|---|---|---|---|---|---|---|
| 0 MON | 4 MON | 0 MON | 4 MON | 0 MON | 4 MON | |
| Basal diet | 97.5 | 97.5 | 97.5 | 97.5 | 97.5 | 97.5 |
| Nitrate source2 | 0 | 0 | 1.25 | 1.25 | 2.5 | 2.5 |
| Monensin,3 mg/L | 0 | 4 | 0 | 4 | 0 | 4 |
| Urea | 1.2 | 1.2 | 0.65 | 0.65 | 0 | 0 |
| Calcium carbonate4 | 1.3 | 1.3 | 0.6 | 0.6 | 0 | 0 |
1EN = encapsulated calcium nitrate (0, 1.25 and 2.5% of diet DM); MON = sodium monensin (0 and 4 mg/L).
2Nitrate source was manufactured by GRASP Ind. & Com. LTDA and EW|Nutrition GmbH. The source of nitrate was the double salt of calcium ammonium nitrate decahydrate [5Ca(NO3)2 ∙ NH4NO3 ∙ 10H2O]; 85.6% DM; 17.6% N, 19.6% Ca, and 71.4% nitrate on a DM basis.
3Monensin sodium (C36H61NaO1) was manufactured by Elanco Animal Health as Rumensin 90; 198 g of active ingredient per kg.
4Calcium carbonate contained 38% Ca.