. 2018 May 25;96(8):3446–3459. doi: 10.1093/jas/sky211

Table 3.

Effects of EN and MON on gas production and nutrient disappearance on DAIRY (50:50 roughage: concentrate) trials¹

	0 EN		1.25 EN		2.5 EN			P value¹
Item	0 MON	4 MON	0 MON	4 MON	0 MON	4 MON	SEM	EN	MON	EN × MON
Dilution rate (mL, d^-1)	1,523	1,526	1,576	1,545	1,518	1,514	27.91	NS	NS	NS
Gas production
Total gas (L)	2.57^b	2.57^b	2.48^b	2.60^b	2.71^a	2.40^c	0.20	0.01	NS	NS
CH₄, mM/d	32.15^a	26.34^b	26.77^b	23.66^c	22.78^c	19.03^d	1.79	<0.01	<0.01	0.03
CO₂, mM/d	75.32	71.64	68.11	75.91	71.58	75.85	5.06	NS	NS	NS
N₂O (×10^–4), mM/d	33^c	37^b	35^b	36^b	39^ab	46^a	3.8	<0.01	NS	NS
Nutrient disappearance (%)
DM	64.90	65.35	65.16	64.66	64.29	64.21	1.36	NS	NS	NS
NDF	27.64	26.64	28.21	28.4	28.56	26.36	1.15	NS	NS	NS
CP	97.63^ab	97.91^a	97.0^b	97.17^ab	96.85^b	96.86^b	0.38	<0.01	NS	NS
Starch	80.78^a	81.12^a	79.92^ab	80.12^a	78.17^b	78.22^b	0.63	<0.01	NS	NS

^{a– d}Within a row, means without a common superscript letter differ, P < 0.05.

¹EN = encapsulated calcium nitrate (0, 1.25, and 2.5% of diet DM); MON = sodium monensin (0 and 4 mg/L).

²NS = nonsignificant; (P > 0.10).