Table 2.
Forward and reverse primers used for real-time PCR
| Gene symbol1 | Primer sequences | Accession no. |
|---|---|---|
| GAPDH | Forward: 5′-CTGTCCACCTTCCAGCAGAT-3′ | NM_001034 |
| Reverse: 5′-TCACCTTCACCGTTCCAGTT-3′ | ||
| PPARγ | Forward: 5′-GCATTTCCACTCCGCACTAT-3′ | AY137204 |
| Reverse: 5′-GGGATACAGGCTCCACTTTG-3′ | ||
| FABP4 | Forward: 5′-AAACTTAGATGAAGGTGCTCTGG-3′ | AJ4160220 |
| Reverse: 5′-CATAAACTCTGGTGGCAGTGA-3′ | ||
| SREBP1 | Forward: 5′-ACTACCACGCCAAGTTCCTG-3′ | JN790254 |
| Reverse: 5′-CGATGCCAATCTCCTCCTT-3′ | ||
| SCD | Forward: 5′-GCCAACAACTCTGCCTTTATG-3′ | GU947654 |
| Reverse: 5′-CACCAATGACTGACCACCTG-3′ | ||
| ACC1 | Forward: 5′-TATGTCCTCCCAAGCATTCC-3′ | X80045 |
| Reverse: 5′-GCCAATCTCATTTCCTCCTG-3′ | ||
| FASN | Forward: 5′-TTCGGAGATTGTTTCACAGC-3′ | AF479289 |
| Reverse: 5′-GGCAGGAGTTCGCTTCAGTA-3′ | ||
| LPL | Forward: 5′-ACACTTGCCACCTCATTCCT-3′ | X68308 |
| Reverse: 5′-GCACCCAACTCTCATACATTCC-3′ |
1 GAPDH, glyceraldehyde-3-phosphate dehydrogenase; PPARγ, peroxisome proliferator-activated receptor gamma; FABP4, adipocyte fatty acid-binding protein; SREBP1, sterol regulatory element binding protein-1; SCD, stearoyl-CoA desaturase; ACC1, acetyl CoA carboxylase; FASN, fatty acid synthase; LPL, lipoprotein lipase.