Figure 4.

Determination of potential key mucosal genera for RFI and cecal mucosal gene expression in pigs ranked on RFI. Covariations between the relative abundances of mucosal bacterial genera (rel. abundances > 0.002) and RFI and relative gene expression in the cecum using sPLS regression. The network is displayed graphically as nodes (genera and expression of genes) and edges (biological relationship between nodes), with the edge color intensity indicating the level of the association: red = positive, and green = negative. Only the strongest pairwise associations were projected, with score threshold depending on the respective association. The score of the association (Pearson’s correlation) is provided in Supplementary Table A3. Relevance networks for (A) standardized RFI (stdRFI), (B) relative MUC2 expression, (C) relative expression of tight-junction proteins, and (D) relative expression of Toll-like receptors. Gene names: MUC2, mucin 2; OCLN, occludin; TLR, Toll-like-receptor; ZO1, zonula occludens-1.