Table 1.
Primer sequences for real-time quantitative PCR
| Gene | Accession number | Forward primer sequence (5′ to 3′) | Reverse primer sequence (5′ to 3′) | Product size (bp) |
|---|---|---|---|---|
| PPARγ | NM_214379.1 | GATTTCTCCAGC ATTTCCA | GCTCTTCGTGG GTTTGTT | 184 |
| C/EBPα | XM_003127015.3 | CAAAGCCAAGAAGTCGGTAGA | ATTGTCACTGGTCAGCTCCA | 148 |
| Pref-1 | DQ309458.1 | CCCATGGAGCTGAATGCCT | TTGCAAATGCACTGCCAGGG | 169 |
| FABP4 | NM_001002817.1 | AATTGGGCCAGGAATTTGAT | TCTTTCCATCCCACTTCTGC | 108 |
| HSL | NM_214315.1 | GAAATGCCACTGACTGCTGA | CATAGGAGATGAGCCTGACGA | 100 |
| ATGL | NM_001098605.1 | AGGACAGCTCCACCAACATC | TTGCACATCTCTCGAAGCAC | 130 |
| p21 | XM_013977858.2 | CATGTGGACCTGTTGCTGTC | AAATCTGTCATGCTGGTCTGC | 122 |
| p16 | AJ242787.1 | GAGGCTTCACAGTCCTGACC | AGGACCACCAAAGTGTCCAG | 179 |
| CDK2 | NM_001285465.1 | AAATTCATGGATGCCTCTGC | GGTTTAAGGTCTCGGTGCAA | 125 |
| CDK4 | NM_001123097.1 | TGGTTACAAGTGGTGGGACA | CTGGAGCACGGTACCAGAGT | 111 |
| CDK6 | XM_013979690.1 | TGTTTCAGCTTCTCCGAGGT | ACTGTAGATGCGGGCAAGAC | 137 |
| Cyclin A | GQ265874.1 | GCAGCAGCCTTTCATTTAGC | GGTGAAGGTCCAGGAGACAA | 118 |
| Cyclin D1 | XM_013994006.1 | AAGTGCGTGCAGAAGGAAAT | AGGAAGCGGTCCAGGTAGTT | 131 |
| GAPDH | NM_001206359.1 | ATGGTCCACATGGCCTCCAA | GAAGTCAGGAGATGCTCGGTG | 142 |