Table 2.
Ingredient composition of experimental diets, as-fed basis
| Ingredient, % | Phytase, FTU1 per kilogram complete feed | |||||
|---|---|---|---|---|---|---|
| PC2 | NC | 500 | 1,000 | 2,000 | 4,000 | |
| Corn | 64.65 | 65.58 | 65.58 | 65.58 | 65.58 | 65.58 |
| Soybean meal | 29.00 | 29.00 | 29.00 | 29.00 | 29.00 | 29.00 |
| Soybean oil | 3.00 | 3.00 | 3.00 | 3.00 | 3.00 | 3.00 |
| Cornstarch | 0.40 | 0.40 | 0.375 | 0.35 | 0.30 | 0.20 |
| Limestone | 0.83 | 0.90 | 0.90 | 0.90 | 0.90 | 0.90 |
| Dicalcium phosphate | 1.00 | – | – | – | – | – |
| L-Lys HCL | 0.12 | 0.12 | 0.12 | 0.12 | 0.12 | 0.12 |
| Titanium dioxide | 0.40 | 0.40 | 0.40 | 0.40 | 0.40 | 0.40 |
| Salt | 0.40 | 0.40 | 0.40 | 0.40 | 0.40 | 0.40 |
| Vitamin-mineral premix3 | 0.20 | 0.20 | 0.20 | 0.20 | 0.20 | 0.20 |
| Phytase premix4 | – | – | 0.025 | 0.05 | 0.10 | 0.20 |
| Total | 100.00 | 100.00 | 100.00 | 100.00 | 100.00 | 100.00 |
1FTU = phytase units.
2PC = positive control; NC = negative control.
3Provided the following quantities of vitamins and micro-minerals per kilogram of complete diet: vitamin A as retinyl acetate, 11,136 IU; vitamin D3 as cholecalciferol, 2,208 IU; vitamin E as DL-α tocopheryl acetate, 66 IU; vitamin K as menadione dimethylprimidinol bisulfite, 1.42 mg; thiamin as thiamine mononitrate, 0.24 mg; riboflavin, 6.59 mg; pyridoxine as pyridoxine hydrochloride, 0.24 mg; vitamin B12, 0.03 mg; D-pantothenic acid as D-calcium pantothenate, 23.5 mg; niacin, 44.1 mg; folic acid, 1.59 mg; biotin, 0.44 mg; Cu, 20 mg as copper sulfate; Fe, 126 mg as iron sulfate; I, 1.26 mg as ethylenediamine dihydriodide; Mn, 60.2 mg as manganous sulfate; Se, 0.25 mg as sodium selenite (0.125 mg) and selenium yeast (0.125 mg); and Zn, 124.9 mg as zinc sulfate.
4The phytase premix (Optiphos, Huvepharma, Sofia, Bulgaria) contained 2,000 units of phytase per gram.