. 2018 Apr 24;96(7):2838–2850. doi: 10.1093/jas/sky156

Table 3.

Primer sequences, accession number, and reference for all gene transcripts analyzed by real-time reverse transcription–PCR

Target gene	Primer sequence	Accession number	Source
Genes of interest
20,50-oligoadenylate synthetase
Forward	ACCCTCTCCAGGAATCCAGT	NM 001040606	Fricke et al. (2016)
Reverse	GATTCTGGTCCCAGGTCTGA	NM 001040606	Fricke et al. (2016)
Cyclooxygenase-2
Forward	TCCTGAAACCCACTCCCAACA	NM_174445	Takagi et al. (2008)
Reverse	TGGGCAGTCATCAGGCACAG	NM_174445	Takagi et al. (2008)
Interferon-stimulated gene 15
Forward	GGTATGAGCTGAAGCAGTT	NM_174366	Fricke et al. (2016)
Reverse	ACCTCCCTGCTGTCAAGGT	NM_174366	Fricke et al. (2016)
Interferon-tau
Forward	GCCCTGGTGCTGGTCAGCTA	AF238612	Rizos et al. (2003)
Reverse	CATCTTAGTCAGCGAGAGTC	AF238612	Rizos et al. (2003)
Myxovirus resistance 2
Forward	CTTCAGAGACGCCTCAGTCG	NM 173941	Fricke et al. (2016)
Reverse	TGAAGCAGCCAGGAATAGTG	NM 173941	Fricke et al. (2016)
Prostaglandin E synthase
Forward	CGCTGCTGGTCATCAAAAT	NM_174443.2	Takagi et al. (2008)
Reverse	GGAAGGGGTAGATGGTCTCC	NM_174443.2	Takagi et al. (2008)
Reference genes
β-Actin
Forward	CTGGACTTCGAGCAGGAGAT	AY141970	Gifford et al. (2007)
Reverse	GGATGTCGACGTCACACTTC	AY141970	Gifford et al. (2007)
β2-Microglobulin
Forward	GGGCTGCTGTCGCTGTCT	NM_173893	Silva et al. (2008)
Reverse	TCTTCTGGTGGGTGTCTTGAGT	NM_173893	Silva et al. (2008)
Glyceraldehyde-3-phosphate dehydrogenase
Forward	ACCCAGAAGACTGTGGATGG	NM_001034034	Cerri et al. (2012)
Reverse	CAACAGACACGTTGGGAGTG	NM_001034034	Cerri et al. (2012)
Ribosomal protein L19
Forward	ATTGACCGCCACATGTATCA	NM_001040516	Monteiro et al. (2014)
Reverse	GCGTGCTTCCTTGGTCTTAG	NM_001040516	Monteiro et al. (2014)
Suppressor of zeste 12 homolog
Forward	GAACACCTATCACACACATTCTTGT	XM_582605	Walker et al. (2009
Reverse	TAGAGGCGGTTGTGTCCACT	XM_582605	Walker et al. (2009
Zinc finger protein 131
Forward	AGAAAGAAGCTTTATGAATGTCAGG	NM_001101218	Walker et al. (2009)
Reverse	GTTTATCTCCAGTGTGTATCACCAG	NM_001101218	Walker et al. (2009)