Table 7.
Expression of genes associated with pregnancy establishment in the endometrium, conceptus, and blood from beef cows supplemented with Ca salts of soybean oil (CSSO) or prilled SFA source (CON) in Exp. 21,2
| Item | CSSO | CON | SEM | P value |
|---|---|---|---|---|
| Endometrium2 | ||||
| Cyclooxygenase-2 | 4.88 | 5.11 | 1.32 | 0.89 |
| Prostaglandin E synthase | 5.76 | 7.40 | 1.10 | 0.30 |
| Conceptus2 | ||||
| Interferon-tau | 21.3 | 12.1 | 3.4 | 0.05 |
| Prostaglandin E synthase | 2.22 | 2.50 | 0.48 | 0.69 |
| Blood cells3 | ||||
| Interferon-stimulated gene 15 | ||||
| Pregnant | 43.1 | 29.8 | 4.6 | 0.04 |
| Nonpregnant | 1.87 | 3.57 | 5.48 | 0.81 |
| Myxovirus resistance 2 | ||||
| Pregnant | 20.2 | 20.1 | 2.7 | 0.98 |
| Nonpregnant | 2.30 | 4.80 | 3.01 | 0.48 |
| 20,50-oligoadenylate synthetase | ||||
| Pregnant | 26.8 | 18.3 | 2.7 | 0.03 |
| Nonpregnant | 1.67 | 2.64 | 3.33 | 0.83 |
1CSSO = daily supplementation (per cow) with 100 g of a soybean meal + 100 g of ground corn + 100 g of Ca salts of soybean oil (Essentiom, Church and Dwight Co., Inc., Princeton, NJ); CON = daily supplementation (per cow) with 100 g of a soybean meal + 100 g of ground corn + 87 g of prilled saturated fat (Energy Booster 100, Milk Specialties, Eden Prairie, MN) + 13 g of limestone. Treatments were offered from day 0 (timed AI) to 21 of the experiment. All results are covariately adjusted to estrus expression (Estrotect; Rockway Inc., Spring Valley, WI; Thomas et al., 2014) from day −3 to 0 of the experiment.
2Conceptus were collected via transcervical flushing, and endometrial biopsy was performed on day 15 from 44 cows (CSSO, n = 20; CON, n = 24). Only samples from cows with a retrieved conceptus were analyzed. Values are expressed as relative fold change compared with threshold cycle of reference genes analyzed within the same sample (Ocón-Grove et al., 2008).
3Blood samples collected from nonflushed cows (CSSO, n = 25; CON, n = 21) into PAXgene tubes (BD Diagnostics, Sparks, MD) for whole-blood RNA extraction on day 20 of the experiment and analyzed according to cow pregnancy status on day 30. Values are expressed as relative fold change compared with threshold cycle of reference genes analyzed within the same sample (Ocón-Grove et al., 2008).