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. 2018 Jun 14;96(8):3121–3130. doi: 10.1093/jas/sky204

Table 3.

Effect of increasing levels of biocarbon on fermentation and gas production in the RUSITEC

Item Level of biocarbon (% of diet DM) P value
0 0.5 1.0 2.0 SEM Linear Quadratic 0 vs. All
pH 6.8 6.9 6.8 6.8 0.04 0.66 0.41 0.49
NH3-N, mmol/d 6.6 7.0 7.1 7.3 0.40 0.06 0.48 0.06
Total VFA, mmol/d 61.0 61.3 64.2 68.5 1.44 0.005 0.54 0.02
VFA, mmol/d
 Acetate (A) 34.7 34.1 36.4 39.0 1.84 0.001 0.38 0.10
 Propionate (P) 14.1 14.6 15.4 16.6 0.45 0.001 0.98 0.008
 Butyrate 7.1 7.7 7.3 8.1 0.49 0.02 0.70 0.06
 BCVFA1 1.76 1.75 1.84 2.0 0.06 0.001 0.29 0.08
 Valerate 2.40 2.62 2.48 2.36 0.58 0.58 0.34 0.63
 Caproate 0.73 0.69 0.68 0.69 0.70 0.45 0.46 0.26
 A:P 2.42 2.35 2.36 2.38 0.08 0.66 0.29 0.24
Gas production, L/d 2.18 1.92 2.15 2.04 0.08 0.63 0.57 0.14
CH4, % of gas 7.4 6.8 6.8 6.9 0.68 0.02 0.03 0.001
CH4, mg/d 110.8 82.8 95.3 90.7 9.23 0.07 0.05 0.002
CH4, g/g DM incubated 11.1 8.3 9.5 8.9 0.92 0.04 0.05 0.001
CH4, g/g DM digested 15.2 11.0 12.8 11.8 1.22 0.06 0.03 0.001
Protozoa, ×104/mL 5.2 4.3 6.1 5.5 0.41 0.21 0.68 0.91

A:P = acetate to propionate ratio.

1Branched-chain volatile fatty acids (isobutyrate + isovalerate).