Table 4.

Effect of increasing levels of biocarbon on nutrient disappearances and microbial N synthesis in the RUSITEC

	Level of biocarbon (% of diet DM)					P value
Item	0	0.5	1.0	2.0	SEM	Linear	Quadratic	0 vs. All
Nutrient disappearance,1 %
DM	73.2	74.8	74.5	75.5	0.88	0.006	0.38	0.006
OM	72.9	75.0	74.6	75.2	2.23	0.005	0.04	0.001
CP	84.3	85.8	86.4	86.5	1.28	0.02	0.10	0.007
NDF	39.9	43.8	44.3	46.4	1.63	0.001	0.09	0.001
ADF	29.9	35.3	35. 5	38.2	1.57	0.001	0.08	0.001
Starch	98.0	98.2	98.0	98.1	0.57	0.83	0.87	0.66
Production of microbial N,2 mg/d
Total	85.7	92.1	95. 8	100.1	8.27	0.01	0.43	0.02
LAB3	56.9	64.6	68.0	72.3	6.61	0.02	0.39	0.02
FPB4	23.3	20. 8	21.4	21.1	2.41	0.19	0.20	0.04
FPA5	5.7	6.4	6.5	6.9	0.58	0.16	0.71	0.20
N fractions,1 mg/100 mL
LPep N6	19.0	16.1	18.9	15.6	1.64	0.27	0.84	0.30
SPep + AA N7	6.2	7.9	7.6	7.6	1.01	0.49	0.43	0.25

¹Samples from days 11 to 15.

²Samples from days 17 to 18.

³Liquid associate bacteria.

⁴Feed particle-bound bacteria.

⁵Feed particle-associated bacteria.

⁶Large peptides.

⁷Small peptides plus AA.